VDT Radiation Effect on Human Total DNA Evaluated by FIGE
M. Chalier (1), Laverdure, A.M., Tritto G., and J. Surbeck
(1) Medical Doctor, ICOH/CIST, A_NOX Laboratory, 41-43 rue de Reuilly, 75012 Paris, France
Presented at the 27th International Congress on Occupational Health (ICOH 2003), Iguassu Falls, Brazil, 23-28 February 2003, FP 39.9., p. 128
AIMS: To evaluate the effect of radiation from video display terminals (VDTs) on human total DNA.
MATERAL AND METHODS: 1 mg of Human Female Umbilical DNA® (Sigma) was embedded into 1% agarose-TBE 1X and stored into 500 ml thick wall Eppendorff (GeneAmp). VDTs are powered by a 50 Hz alternating voltage of 220V. Measured electric and magnetic fields were 13 V/M and 50 nT, respectively. 8 samples were exposed to VDT during 24 h at room temperature (20°C) in dark. As control, 9 samples were not exposed and left in the dark at room temperature. 8 samples were exposed in front of a VDT equipped by EMF-Bioshield®, an experimental VDT radiation protector. The agarose plugs were put on a gel electrophoresis (agarose 1%-TBE 0.5 X) to perform field inversion gel electrophoresis (FIGE). Molecular weight markers were DNA Mix Digest® (Sigma) and HindIII Echo RI® (Appligene). The Hoefer® PC 500 Switch Back PulseTM Controlled was programmed for single phase runs (run 18 hours, reverse ratio 3/1, pulse time 0.1-0.7 s, 6.5 volts/cm). The gel was revealed into BET solution (0.05 mg.ml) during 30 minutes and rinsed 30 minutes into distilled water. Gels were photographied under UV exposure. Densitometric profiles were done using NIH Image 1.62 Software.
RESULTS: All the unexposed and exposed-protected DNA samples showed the same profile.. In the case of all exposed DNA samples, there is a shift greater than 10Kb to lower molecular weight of the DNA smears.
CONCLUSION: With special attention to sensibility and specificity of the method, these facts could be interpretated as: 1 - the VDTs radiation could break DNA and 2 - the experimental system EMF-Bioshield® tested seemed to be able to ensure good preservation of the DNA exposed to the VDT radiation.